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Separation and characterization of metallothionein in two species of seals by high performance liquid chromatography-atomic absorption spectrophotometry |
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| Authors: | Y Mochizuki K T Suzuki H Sunaga T Kobayashi R Doi |
| Abstract: | Metallothionein (MT) was separated from the livers of two species of seals, ribbon seal (Histriophoca fasciata) and harbour seal (Phoca vitulina), by high performance liquid chromatography--atomic absorption spectrophotometry (HPLC-AAS). Both gel permeation and anion-exchange HPLC demonstrated that MT was present as a mixture of at least two isoforms in the livers of both species of seals. |
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