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Restriction fragment length polymorphism and chromosome mapping of a mouse homeo box gene,Hox-2.1 |
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| Authors: | Tomomasa Watanabe Shigeo Masaki Naoki Takahashi Masahiko Nishimura Hideki Kato |
| Institution: | 1. Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan 2. Department of Biochemistry, Faculty of Medicine, University of Tokyo, 113, Hongo, Bunkyoku, Tokyo, Japan 3. Institute for Experimental Animals, Hamamatsu University School of Medicine, Handacho, 431-31, Hamamatsu, Japan 4. Department of Animal Genetics, Central Institute for Experimental Animals, Nogawa, Miyamaeku, 213, Kawasaki, Japan |
| Abstract: | Restriction endonuclease fragment length polymorphisms (RFLPs) were found using the cDNA probe Hox-2.1 for the homeo box-2.1 gene in the mouse. Polymorphism was detected in restriction patterns generated by fragments fromHindIII digestion. The great majority of laboratory strains of mice carries theHox-2.1 a allele. Only two laboratory strains carry theHox-2.1 b allele. Among strains of wild origin, the European subspecies (Mus m. domesticus, M. m. brevirostris, andM. m. musculus) and some Asian subspecies (M. m. castaneus) carry theHox-2.1 a allele. The subspecies from Far Eastern countries (M. m. molossinus, Chinese mice of wild origin, andM. m. yamashinai) carry theHox-2.1 ballele. Using the RFLP, theHox-2.1 gene was mapped on chromosome 11. Three-point cross test data showed that the recombination frequency is 29.6% between theHba and theHox-2.1 genes and 23.5% between theHox-2.1 and theEs-3 genes. The gene order ofHba-Hox-2.1-Es-3 has been confirmed. |
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