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Application of Coriphosphine Staining to the Study of the Macronuclear Anlage of Stylonychia mytilus
Authors:CONCEPIÓN FEDRIANI  ANTONIO TORRES  JULIO PÉREZSILVA
Institution:Departamento de Microbiologia de la Facultad de Ciencias de la Universidad de Scvilla, Spain
Abstract:SYNOPSIS. Coriphosphine staining of Stylonychia mytilus exconjugants at different times after separation reveals some details of the developing macronucleus. Green fluorescence is seen in both bands and heterochromatic blocks of the polytene chromosomes. No red fluorescence was observed along these chromosomes. Fragments of the old macronucleus and the pycnotic micronuclei have red or orange fluorescence. Red fluorescence is characteristic also of nucleoli in the new macronucleus.
Keywords:Stylonychia mytilus            macronuclear anlage  coriphosphine staining  fluorescence microscopy
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