Uptake and inhibition kinetics of nitrogen in Microcystis aeruginosa: Results from cultures and field assemblages collected in the San Francisco Bay Delta,CA |
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| Affiliation: | 1. Department of Biology, Clarkson University, Potsdam 13699, NY, USA;2. Department of Biology, Central Michigan University, Mount Pleasant 48859, MI, USA;3. Institute for Great Lakes Research, Central Michigan University, Mount Pleasant 48859, MI, USA;1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;2. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;1. Department of Environmental Sciences and Lake Erie Center, University of Toledo, 6200 Bayshore Dr., Oregon, OH 43616, United States;2. Department of Biological Sciences, Kent State University, 256 Cunningham Hall, Kent, OH 44242, United States;1. Department of Earth, Environmental, and Planetary Sciences, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-7216, USA;2. Department of Environmental Sciences, University of Toledo, Lake Erie Center, 6200 Bayshore Rd., Oregon, OH 34616, USA;3. Department of Civil Engineering, Chemical and Environmental Engineering, School of Green Chemistry, University of Toledo, 2801 W. Bancroft St. Toledo, OH 43606, USA;4. Department of Biology, Sustainability Sciences Institute, University of Wisconsin–Stout, Department of Biology, 123E Jarvis Hall, Menomonie, WI 54751, USA;5. National Center for Water Quality Research, Heidelberg University, 310 E Market St., Tiffin, OH 44883, USA;6. Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, M1C 1A4, Canada;7. U.S. Geological Survey, Great Lakes Science Center, 1451 Green Rd., Ann Arbor, MI 48105, USA;8. The Ohio State University, Franz Theodore Stone Laboratory Ohio Sea Grant, P.O. Box 119, 878 Bayview Ave., Put-in-Bay, OH 43456, USA |
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| Abstract: | The nitrogen (N) uptake kinetic parameters for Microcystis field assemblages collected from the San Francisco Bay Delta (Delta) in 2012 and non-toxic and toxic laboratory culture strains of M. aeruginosa were assessed. The 15N tracer technique was used to investigate uptake of ammonium (NH4+), nitrate (NO3−), urea and glutamic acid over short-term incubations (0.5–1 h), and to study inhibition of NO3−, NH4+ and urea uptake by NH4+, NO3− and NH4+, respectively. This study demonstrates that Delta Microcystis can utilize different forms of inorganic and organic N, with the greatest capacity for NH4+ uptake and the least for glutamic acid uptake, although N uptake did not always follow the classic Michaelis–Menten hyperbolic relationship at substrate concentrations up to 67 μmol N L−1. Current ambient N concentrations in the Delta may be at sub-saturating levels for N uptake, indicating that if N loading (especially NH4+) were to increase, Delta Microcystis assemblages have the potential for increased N uptake rates. Delta Microcystis had the highest specific affinity, α, for NH4+ and the lowest for NO3−. In culture, N uptake by non-toxic and toxic M. aeruginosa strains was much higher than from the field, but followed similar N utilization trends to those in the field. Neither strain showed severe inhibition of NO3− uptake by NH4+ or inhibition of NH4+ uptake on NO3−, but both strains showed some inhibition of urea uptake by NH4+. |
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| Keywords: | Nitrogen uptake Inhibition kinetics Ammonium Cyanobacteria San Francisco Bay Delta |
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