Determination of N-acetylglucosaminyltransferases III, IV and V in normal and hepatoma tissues of rats |
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Authors: | A Nishikawa J Gu S Fujii N Taniguchi |
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Institution: | Department of Biochemistry, Osaka University Medical School, Japan. |
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Abstract: | N-Acetylglucosaminyltransferase III, IV and V activities were assayed in various rat tissues and hepatomas using the same fluorescence-labeled sugar chain, GlcNAc beta 1-2Man alpha 1-3-(GlcNAc beta 1-2Man alpha 1-6)Man beta 1-4GlcNAc beta 1-4GlcNAc-2-aminopyridine as a substrate. The N-acetylglucosaminyltransferase III activity toward the substrate is the highest in most rat tissues including primary rat hepatoma. A relatively higher activity for GnT-V is found in small intestine, serum and hepatoma as compared to that of GnT-IV. Some kinetic properties of these enzymes in crude extracts were also determined. |
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