Identification of microorganisms using random primed PCR |
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Authors: | Alan J Mileham |
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Institution: | (1) Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP Cambridge, UK |
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Abstract: | The polymerase chain reaction has facilitated the use of molecular approaches in microbiology including new strategies for
the rapid identification of micro-organisms. Approaches based on the use of random primers and standard conditions, allows
characteristic DNA fingerprints to be generated from any micro-organism even in the absence of information about its DNA sequence.
Different primers can be used to produce genus-specific, species-specific, or even strain-specific DNA fingerprints. This
article covers the background to this strategy, describes three different approaches to generating DNA fingerprints using
random primers, and provides experimental detail for one method, RAPD. |
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Keywords: | RAPD PCR DNA fingerprint identification random priming |
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