<Emphasis Type="Italic">Sporotalea propionica</Emphasis> gen. nov. sp. nov., a hydrogen-oxidizing,oxygen-reducing,propionigenic firmicute from the intestinal tract of a soil-feeding termite |
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Authors: | Hamadi I Boga Rong Ji Wolfgang Ludwig Andreas Brune |
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Institution: | 1.Lehrstuhl Mikrobielle ?kologie, Fachbereich Biologie,Universit?t Konstanz,Konstanz,Germany;2.Lehrstuhl für Mikrobiologie,Technische Universit?t München,München,Germany;3.Max Planck Institute for Terrestrial Microbiology,Marburg,Germany;4.Botany Department,Jomo Kenyatta University of Agriculture and Technology,Nairobi,Kenya;5.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment,Nanjing University,Nanjing,China |
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Abstract: | An unusual propionigenic bacterium was isolated from the intestinal tract of the soil-feeding termite Thoracotermes macrothorax. Strain TmPN3 is a motile, long rod that stains gram-positive, but reacts gram-negative in the KOH test. It forms terminal
endospores and ferments lactate, glucose, lactose, fructose, and pyruvate to propionate and acetate via the methyl-malonyl-CoA
pathway. Propionate and acetate are formed at a ratio of 2:1, typical of most propionigenic bacteria. Under a H2/CO2 atmosphere, the fermentation product pattern of glucose, fructose, and pyruvate shifts towards propionate formation at the
expense of acetate. Cell suspensions reduce oxygen with lactate, glucose, glycerol, or hydrogen as electron donor. In the
presence of oxygen, the product pattern of lactate fermentation shifts from propionate to acetate production. 16S rRNA gene
sequence analysis showed that strain TmPN3 is a firmicute that clusters among the Acidaminococcaceae, a subgroup of the Clostridiales
comprising obligately anaerobic, often endospore-forming bacteria that possess an outer membrane. Based on phenotypic differences
and less than 92% sequence similarity to the 16S rRNA gene sequence of its closest relative, the termite hindgut isolate Acetonema longum, strain TmPN3T is proposed as the type species of a new genus, Sporotalea
propionica gen. nov. sp. nov. (DSM 13327T, ATCC BAA-626T). |
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Keywords: | Acidaminococcaceae Propionate formation Oxygen reduction Hydrogen oxidation Lactate fermentation Termite gut |
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