Two tunicamycin-sensitive components involved in agglutination and fusion ofChlamydomonas reinhardtii gametes |
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Authors: | Yoshihiro Matsuda Kazuko Sakamoto Naoko Kiuchi Tsuguo Mizuochi Yoshihiro Tsubo Akira Kobata |
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Institution: | (1) Department of Biology, Faculty of Science, Kobe University, Nada-ku, 657 Kobe, Japan;(2) Department of Biochemistry, Kobe University School of Medicine, Chuo-ku, 650 Kobe, Japan;(3) Department of Biology, College of Liberal Arts, Kobe University, Nada-ku, 657 Kobe, Japan |
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Abstract: | To find out glycoproteins involved in the mating reaction ofChlamydomonas reinhardtii, the effect of tunicamycin (TM), a potent inhibitor of glycosylation of proteins, was studied. TM, when present during gametogenesis, blocked the acquisition of agglutinability ofmt
+ cells. TM also inhibited the recovery of agglutinability ofmt
+ gamete after trypsin treatment. On the contrary, TM blocked neither the acquisition of agglutination during gametogenesis ofmt
- cells nor the recovery of their agglutinability after trypsinization. It was found, however, that the TM-treatedmt
- gametes can agglutinate but do not fuse with non treatedmt
+ gametes at all. When gametes of gam-1mt
-, a conditional mutant strain for cell fusion, were induced at non permissive temperature of 35°C and then transferred to 25°C, the ability of cell fusion was acquired after about 5 h incubation. Presence of TM completely blocked this acquisition. Based on these evidence, we conclude that at least two TM-sensitive glycoproteins are included in the mating reaction. The first component is located on the flagellar surface ofmt
+ gamete and responsible for agglutination withmt
- flagella. The second component occurs on the surface ofmt
- gamete and plays a role in the fusion withmt
+ gamete.Abbreviations CHI
cycloheximide
-
mt
mating type
- TM
tunicamycin |
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Keywords: | Agglutination Chlamydomonas reinhardtii Fusion Glycoproteins Mating reaction Mutant Trypsinization Tunicamycin |
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