A CHO strain producing high-level human IL-6 with the 3' deletion construct. |
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| Authors: | N Tonouchi N Koyama K Miwa |
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| Affiliation: | Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan. |
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| Abstract: | A Chinese Hamster Ovary (CHO) strain producing high-level human interleukin 6 (IL-6) was obtained, by introduction of the IL-6 cDNA from which the 3' AU-rich region was deleted. The IL-6 mRNA of this strain was stable. The productivity was more than 15 times higher than the previously obtained clone, which has intact IL-6 cDNA in the same expression vector. This strain produced IL-6 at a high level of 30 micrograms ml-1 in batch culture, or a continuous 20 micrograms per 10(6) cells per day in microcarrier cultivation. |
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