| 瘦素对大鼠主动脉血管平滑肌细胞增殖和移行的影响 |
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| 引用本文： | 冯友梅,陈娟,周洁,张雯,王咏雪,曾和松. 瘦素对大鼠主动脉血管平滑肌细胞增殖和移行的影响[J]. 中国组织化学与细胞化学杂志, 2006, 15(4): 361-365 |
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| 作者姓名： | 冯友梅 陈娟 周洁 张雯 王咏雪 曾和松 |
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| 作者单位： | 1. 华中科技大学同济医学院生物化学与分子生物学系,武汉,430030
2. 华中科技大学同济医学院附属同济医院心血管内科,武汉,430030 |
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| 基金项目： | 中国科学院资助项目;全国高等学校博士学科点科研项目 |
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| 摘 要： | 目的本实验旨在研究瘦素(Leptin)对大鼠主动脉血管平滑肌细胞(VSMC)增殖和移行的影响。方法采用原代细胞培养方法建立VSMC细胞模型;以四甲基偶氮唑盐(MTT)比色法、免疫细胞化学染色等方法观察瘦素对VSMC增殖的影响;采用细胞移行实验观察瘦素对VSMC移行的影响;采用MAPK的抑制剂SB203580观察瘦素对VSMC增殖的抑制作用以探讨MAPK在瘦素促VSMC增殖中的信号转导机理。结果瘦素能显著促进VSMC增殖和移行,而MAPK的抑制剂SB203580可显著抑制瘦素对VSMC的增殖效应。结论瘦素具有明显的促VSMC增殖和移行的作用,其作用机制与激活MAPK信号途径有关。
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| 关 键 词： | 瘦素 增殖 移行 丝裂原激活的蛋白激酶 血管平滑肌细胞 |
| 收稿时间： | 2005-04-16 |
| 修稿时间： | 2006-03-13 |
EFFFECTS OF LEPTIN ON THE PROLIFERATION AND MIGRATION OF RAT AORTIC SOMMTH MUSCLE CELLS |
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| Feng Youmei,Chen Juan,Zhou Jie,Zhang Wen,Wang Yongxue,Zeng Hesong. EFFFECTS OF LEPTIN ON THE PROLIFERATION AND MIGRATION OF RAT AORTIC SOMMTH MUSCLE CELLS[J]. Chinese Journal of Histochemistry and Cytochemistry, 2006, 15(4): 361-365 |
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| Authors: | Feng Youmei Chen Juan Zhou Jie Zhang Wen Wang Yongxue Zeng Hesong |
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| Affiliation: | 1Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;2Department of Cardiology, Tongji hospital, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective To investigatethe effect of leptin on the proliferation and migration of vascularsmooth muscle cells (VSMC). Methods By using primary cell culture, MTT colorimetry and immunocytochemistry, we detected the effects of leptin on VSMC proliferation. By using cell migration assay, we detected the effects of leptin on VSMC migration. We also detected the signal transduction pathway of leptin in the proliferation and migration of VSMC by using the specific inhibitor of MAPK (SB203580). Results Leptin stimulated both proliferation and migration of VSMC. Leptin activatedmitogen-activated protein (MAP) kinases.Further more,the MAPKinhibitor SB203580inhibited the proliferative and migratory effectsof leptin. Conclusion These results demonstratethat leptin might be one of the proliferative and migratory factorsfor VSMCand related to the MAPK signal pathway. |
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| Keywords: | Leptin Proliferation Migration MAPK Vascular smooth muscle cells |
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