Polymorphism of sequences and the secondary structure of b/c intron of mitochondrial gene <Emphasis Type="Italic">nad1</Emphasis> in <Emphasis Type="Italic">Monotropa hypopitys</Emphasis> and related ericaceae species |
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Authors: | M A Filyushin N M Reshetnikova E Z Kochieva K G Skryabin |
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Institution: | 1.Bioengineering Institute, Research Center of Biotechnology,Russian Academy of Sciences,Moscow,Russia;2.Tsitsin Main Botanical Garden,Russian Academy of Sciences,Moscow,Russia;3.Department of Biotechnology,Moscow State University,Moscow,Russia |
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Abstract: | Nucleotide sequences of b/c intron of the mitochondrial gene nad1 were determined for the first time and analyzed in 24 plants of Monotropa hypopitys and in three related Ericaceae species. Two mitotypes of b/c intron of the nad1 gene, differing in sequence lengths and the presence of a number of single nucleotide substitutions and indels, were revealed in M. hypopitys. For the first time, a possible pre-mRNA secondary structure of M. hypopitys b/c intron was determined and IBS/EBS binding sites and the borders of six functional domains were identified. |
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