| 引物间同源性和裂口对PCR扩增的影响 |
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| 引用本文： | 周鹏.引物间同源性和裂口对PCR扩增的影响[J].生命科学研究,2000,4(1):35-40. |
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| 作者姓名： | 周鹏 |
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| 作者单位： | 中国热带农业科学院,热带作物生物技术国家重点实验室,中国海南,海口,571101 |
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| 基金项目： | 海南省自然科学基金资助课题之—(39605) |
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| 摘 要： | 采用ＰＣ／Ｇｅｎｅ软件对能扩增出特异睡不能扩增的ＰＣＲ引物进行同源性比较，同源间“裂口（ｇａｐｓ）”差数的统计，得出源率小于或等于３５％，“裂口”差数大于或等于３，是获得理想ＰＣＲ扩增效果的关键参数之一的结论，为引物设计提供了一条直观的依据。
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| 关 键 词： | 引物同源率 “裂口”差数 引物设计 PCR扩增 |
| 文章编号： | 1007-7847(2000)01-0035-06 |
| 修稿时间： | 1999-09-17 |
Effects of Homologization between Primers and Gaps on PCR Amplification |
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| ZHOU Peng.Effects of Homologization between Primers and Gaps on PCR Amplification[J].Life Science Research,2000,4(1):35-40. |
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| Authors: | ZHOU Peng |
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| Institution: | ZHOU Peng
;(National Key Biotechnology Laboratory for Tropical Crops, SCATC, Haikou 571101, Hainan, China) |
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| Abstract: | Homologous rate, which was less than 35% (including 35%), and gaps between primers, which was more than 3 (including 3), were the key factors of an ideal
PCR amplification, by using PC/Gene software to analyze the homologization of amplified or unamplified PCR primers. This conclusion provided a straight basis for primers'
designation. |
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| Keywords: | PCR primers homologious rate gaps PC/Gene software |
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