Cloning and mapping of cat (Felis catus) immunoglobulin and T-cell receptor genes |
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| Authors: | K-W Cho Hwa-Young Youn Masaru Okuda Hitoshi Satoh Stanley Cevario Stephen J O’Brien Toshihiro Watari Hajime Tsujimoto Atsuhiko Hasegawa |
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| Institution: | (1) The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Veterinary Internal Medicine, Bunkyo-ku, Tokyo 113, Japan, JP;(2) Seoul National University, College of Veterinary Medicine, Department of Veterinary Internal Medicine, Shinlim-dong, Seoul 151-742, Korea, KR;(3) The University of Tokyo, The Institute of Medical Science, Department of Pathology, Minato-ku, Tokyo 108, Japan, JP;(4) National Cancer Institute, Laboratory of Genomic Diversity, Frederick, Maryland 21702-1201, USA, US |
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| Abstract: | Molecular cloning and chromosomal mapping of the cat immunoglobulin (Ig) and T-cell receptor (TcR) genes were carried out to provide basic information for genetic analysis of immunologic diseases including leukemias and
lymphomas in cats. We cloned two Ig constant genes, IGHM and IGHG and three TcR constant genes, TRAC, TRGC, and TRDC, by polymerase chain reaction (PCR) amplification of cDNA from cat peripheral blood mononuclear cells. For chromosomal mapping
of the Ig and TcR loci including the IGK, IGL, and TRB on the cat genome, we performed PCR screening of DNAs from 37 cat × rodent somatic cell hybrids by using specific primers
for the given genes. Consequently, three loci for IGH, TRA, and TRD, and two loci for TRB and TRG were found to be syntenic and assigned to cat chromosomes (FCA) B3 and A2, respectively. Further, IGK and IGL loci were mapped on FCA A3 and D3, respectively. These findings support the notion that the genetic linkages between the
Ig and TcR genes are extensively conserved between humans and cats.
Received: 18 June 1997 / Revised: 12 August 1997 |
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| Keywords: | Cat Ig TcR Cloning Mapping |
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