| Binding of Cry δ-endotoxins to brush border membrane vesicles of Helicoverpa armigera and Helicoverpa punctigera （Lepidoptera： Noctuidae） |
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| 作者姓名： | CHUNYAN LIAO LIZ BROOKS STEPHEN C. TROWELL RAYMOND J. AKHURST |
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| 作者单位： | [1]CSIRO Entomology,Australian National University, ACT, Australia [2]Division of Biochemistry and Molecular Biology, Australian National University, ACT, Australia |
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| 摘 要： | The relatively low susceptibility ofHelicoverpa armigera to CrylAc, its history of resistance to chemical insecticides and the seasonal decline in expression of CrylAc in transgenic cotton necessitated the development of cotton expressing two insecticidal proteins to provide sustainable control of this multinational pest. To manage the resistance issue, it was essential that the second insecticidal protein have a significantly different mode of action to CrylAc. A common feature of resistance to CrylA proteins in several species as well as H. armigera has been a change in the binding site. A study of binding sites for some Cry proteins in the brush border membrane vesicles （BBMV） ofH. armigera and Helicoverpa punctigera was undertaken. The binding affinity for CrylAc was higher than for CrylAb, matching their relative toxicities, and CrylAc and CrylAb were found to share at least one binding site in both I-1. armigera and I-1. punctigera. However Cry2Aa did not compete with CrylAc for binding and so could be used in transgenic cotton in combination with CrylAc to control H. armigera and manage resistance. Variation in the susceptibilities of three different H. armigera strains to CrylAc correlated with the parameter Bmax/Kcom.
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| 关 键 词： | δ-内毒素 囊膜 鳞翅类 昆虫学 Helicoverpa punctigera 棉铃虫 |
Binding of Cry δ-endotoxins to brush border membrane vesicles of Helicoverpa armigera and Helicoverpa punctigera (Lepidoptera: Noctuidae) |
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| Authors: | CHUNYAN LIAO LIZ BROOKS STEPHEN C TROWELL RAYMOND J AKHURST |
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| Institution: | CSIRO Entomology, Australian National University, ACT, Australia;, and Division of Biochemistry and Molecular Biology, Australian National University, ACT, Australia |
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| Abstract: | Abstract The relatively low susceptibility of Helicoverpa armigera to Cry 1 Ac, its history of resistance to chemical insecticides and the seasonal decline in expression of Cry 1 Ac in transgenic cotton necessitated the development of cotton expressing two insecticidal proteins to provide sustainable control of this multinational pest. To manage the resistance issue, it was essential that the second insecticidal protein have a significantly different mode of action to Cry1 Ac. A common feature of resistance to Cry1 A proteins in several species as well as H. armigera has been a change in the binding site. A study of binding sites for some Cry proteins in the brush border membrane vesicles (BBMV) of H. armigera and Helicoverpa punctigera was undertaken. The binding affinity for Cry1Ac was higher than for Cry1Ab, matching their relative toxicities, and Cry1Ac and Cry1Ab were found to share at least one binding site in both H. armigera and H. punctigera. However Cry2Aa did not compete with Cry 1 Ac for binding and so could be used in transgenic cotton in combination with Cry 1 Ac to control H. armigera and manage resistance. Variation in the susceptibilities of three different H. armigera strains to Cry 1 Ac correlated with the parameter Bmax/Kcom. |
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| Keywords: | Helicoverpa armigera Helicoverpa punctigera Bacillus thuringiensis δ-endotoxin brush border membrane vesicles ligand binding |
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