Amplified fragment length polymorphism (AFLP) analysis of Listeria monocytogenes |
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| Authors: | Guerra M M Bernardo F McLauchlin J |
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| Institution: | 1. CIISA / Laboratório de Inspecçāo Sanitária, Faculdade de Medicina Veterinária Universidade Técnica de Lisboa, Portugal;2. Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, Public Health Laboratory Service Central Public Health Laboratory, UK;1. Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore;2. College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China;3. St. John''s Island National Marine Laboratory, National University of Singapore, 119279, Singapore;4. Asian School of the Environment, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore;5. Department of Chemical and Materials Engineering, Nazarbayev University, 010000, Astana, Kazakhstan;6. The Environment & Resource Efficiency Cluster (EREC), Nazarbayev University, 010000, Astana, Kazakhstan;1. Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India;2. Laboratório de Análises Cromatográficas e flavour – LAF, Universidade Federal de Sergipe, CEP 49100-000, Brazil;3. Department of Microbial Engineering, Konkuk University, Seoul 143-701, Republic of Korea;1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, Guangdong, China;2. Centre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand;1. Institute of Hydrogen Economy, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Malaysia;2. Process System Engineering Centre (PROSPECT), Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Malaysia;3. Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Malaysia;1. College of Computer Science, Chongqing University, Chongqing 400044, PR China;2. Department of Mathematics, Texas A & M University at Qatar, Qatar |
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| Abstract: | An agarose gel based single enzyme AFLP method using EcoR1 digestion of Listeria monocytogenes DNA was developed for epidemiological typing. The method was evaluated with 84 L. monocytogenes cultures, and results were compared with those obtained with serotyping, phage-typing and cadmium and arsenic resistance typing. The AFLP method was reproducible and 14 different banding patterns comprising between five and eight DNA fragments were produced. All except two of the AFLP patterns were serorype specific. Different AFLP patterns were recognised within serovar 4b (four patterns), 1/2a (two patterns), 1/2b (six patterns): single patterns were obtained from cultures of serovars 1/2c, 3a, 3b and 3c. There were associations with AFLP results and those from phage-typing and cadmium and arsenic resistance typing, although each method showed some independence. This preliminary evaluation suggests that this AFLP method will be useful for epidemiological typing of L. monocytogenes. |
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