In vitro micropropagation and ex vitro acclimation of Bupleurum kaoi-an endangered medicinal plant native to Taiwan |
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Authors: | Uei-Chern Chen Chi-Ni Hsia Mau-Shing Yeh Dinesh Chandra Agrawal Hsin-Sheng Tsay |
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Institution: | (1) Agronomy Division, ARI, Wufong, 413 Taichung, Taiwan;(2) Department of Agronomy, National Chung Hsing University, 402 Tachung, Taiwan;(3) Graduate Institute of Biotechnology, Chaoyang University of Technology, Wufong, 413 Taichung, Taiwan |
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Abstract: | Summary This study reports an improved protocol for in vitro-shoot multiplication and ex vitro acclimation of Bupleurum kaoi, an endangered medicinal herb. Nodal segments were cultured in half-strength Murashige and Skoog (MS) basal medium supplemented
with different concentrations of benzyladenine (BA) and kinetin. The presence of 0.25 mg l−1 BA induced the highest number of shoots per explant after 8 wk of culture. Although BA was more effective than kinetin on
shool multiplication, it induced hyperhydric shoots at all concentrations tested. The use of dispense paper (DP) instead of
aluminum foil (AF) for container closure was found to reduce hyperhydricity and improve ex vitro acclimation. The best survival rate (61%) was obtained when plantlets were grown in MS basal medium containing 0.5 mg l−1 indole-3-butyric acid and 0.1–0.2 mg l−1 α-naphthaleneacetic acid using DP as container closure. Leaves of the plant treated with AF6 (two layers of AF as container
closure and 6 wk of incubation) lacked epicuticular wax and possessed larger stomata, higher stomata density, and fewer functional
stomata compared to those of plants treated with AF2+DP4 (two layers of AF for 2 wk, then replaced AF by three layers of DP
for 4wk) and ex vitro-acclimated plantlets. |
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Keywords: | acclimation Bupleurum kaoi container closure hyperhydricity proliferation rooting scanning electron microscope |
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