Le ionophore A23187 |
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Authors: | P. Fenichel M. Donzeau BL. Hsi N. Ayraud |
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Affiliation: | 1. INSERM U210-NICE, 06034 Cedex, Nice, France 2. Laboratoire de Biologie de la Reproduction, Faculté de Médecine-NICE, 06034 Cedex, Nice, France
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Abstract: | The acrosomic status of spermatozoa prepared for IVF has been evaluated by means of immunofluorescence test from Fenichel and Hsi using calcium A 23187 ionophore as inductor of acrosome reaction (AR). The spontaneous AR remains slight, even after 6 hour-incubation in Menezo B2 (6,8+2,7%). The response to ionophore, moderate before (11,2+9%), frankly increases after a 6h-capacitation (28,9+8,3%) in a group of 25 IVF couples (tubal indication, normal semen, positive fertilization). Nevertheless, it remains slight or null in 4 cases of unexplained repeated failure of fertilization. The response to ionophore A 23187 allows to explore the kinetics of capacitation of spermatozoa and their ability to perform AR. Its significance in terms of fecondance remains to be precised. |
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