黑水虻幼虫蛋白及其酶解产物的抗氧化活性研究

为了探究黑水虻Hermetia illucens幼虫蛋白及酶解产物的抗氧化活性，以鲜活黑水虻幼虫冻虫为原料，采用碱提酸沉法提取黑水虻蛋白，通过碱性蛋白酶、菠萝蛋白酶、风味蛋白酶、木瓜蛋白酶对其蛋白质溶液进行酶解，分别从2，2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基、羟自由基、1,1-二苯基-2-三硝基苯肼（DPPH）自由基3个方面对黑水虻蛋白及其酶解液的抗氧化能力进行测定。结果表明：黑水虻幼虫蛋白及4种酶解后的蛋白肽具有较好的抗氧化活性，其中黑水虻幼虫蛋白对ABTS自由基、羟自由基、DPPH自由基的半抑制浓度（IC50）分别为2.91、0.232、0.764 mg/mL，而酶解过后的蛋白肽具有更强的抗氧化活性，对ABTS自由基、羟自由基、DPPH自由基的最低半抑制浓度（IC50）分别为0.295、0.082、0.354 mg/mL。本研究初步证明了酶解制备抗氧化肽的可行性，为昆虫蛋白资源的利用和无抗饲料的研制提供了新的研究思路。

Antioxidant activity of protein and its enzymatic hydrolysate from the black soldier fly (Hermetia illucens) larvae

In order to explore the antioxidant activity of protein and enzymatic hydrolysates of black water fly larvae, the protein of black water fly larvae was extracted by alkali extraction and acid precipitation. The protein solution was hydrolyzed by alkaline protease, bromelain, flavourzyme and papain Hydroxyl radical, 1,1- two phenyl -2- three nitro phenyl hydrazine (DPPH) radical three aspects of the black water fly protein and its enzymatic hydrolysate antioxidant capacity were determined, and polypropylene gel electrophoresis for molecular weight determination. The results showed that: The protein and four kinds of protein peptides of black water fly larvae had good antioxidant activity. The half inhibitory concentration (IC50) of the protein of black water fly larvae to ABTS free radical, hydroxyl free radical and DPPH free radical were 2.91, 0.232 and 0.764 mg/mL, respectively. The protein peptides of black water fly larvae had stronger antioxidant activity to ABTS free radical, hydroxyl free radical and DPPH free radical The lowest half inhibitory concentrations (IC50) of the three groups were 0.295, 0.082 and 0.354 mg/mL. This study preliminarily proved the feasibility of preparing antioxidant peptides by enzymatic hydrolysis, and provided a new research idea for the utilization of insect protein resources and the development of non-resistant feed.