拟南芥茎顶端分生组织相关突变体的表型与结构分析

以拟南芥野生型(C24)和T-DNA插入诱发的突变体(155系)为材料,通过表型分析、组织切片、GUS基因表达的组织化学定位等研究方法对155系的形态结构和生长发育进行了较为细致的观察分析,结果发现:(1)T-DNA插入诱发的155系突变体植株矮化,叶片等器官体积减小,营养生长阶段延长,发育较C24缓慢;(2)同一时期155系的茎顶端分生组织面积较C24减小,顶端平坦,细胞层数减少,两侧叶原基基部之间的距离缩短,呈现出发育迟缓、从茎顶端分生组织向花分生组织转变延迟等特征;(3)GUS基因特异性地在155系茎顶端分生组织和维管组织中表达.结果表明,T-DNA诱捕基因可能在茎顶端分生组织中发挥作用,由于T-DNA的插入使该基因的功能受到了影响,进而影响了155系中茎顶端分生组织的发育模式,产生了155系的一系列表型改变.

Phenotypical and Structural Characterization of the Arabidopsis Mutant Involved in Shoot Apical Meristem

An Arabidopsis mutant(line 155) induced by T-DNA insertion was studied carefully in phenotypes,microstructures of shoot apical meristem(SAM) and histo-chemistrical localization of GUS gene through comparison with wild type.The results showed that(1) the mutant line 155 exhibited a dwarf phenotype with smaller organs(such as smaller leaves,shorter petioles),slower development and flowering time compared to wild type;(2)Line 155 had a smaller and more flattened SAM with reduced cell layers and shortened distant between two leaf primordial relative to wild type;(3)GUS gene specifically expressed in the SAM and the vascular tissue of the mutant,suggesting that the gene trapped by T-DNA may function in the SAM,and T-DNA insertion could influence the functional activity of the gene in the mutant and lead to the alterations of the SAM and a series of phenotypes in the mutant.