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The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids
Authors:R. Brent Davey   Phillip I. Bird   Suzanne M. Nikoletti   Judyta Praszkier  James Pittard
Affiliation:Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia
Abstract:The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their presence in strains being monitored on galactose fermentation indicator media.
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