Sequence analysis and HLA-DR genotyping of a novel HLA-DRw14 allele |
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Authors: | Fumiya Obata Ichiro Ito Koichi Ito Akio Abe Midori Ohkubo Keiji Onda Yong-guang Yang Koji Watanabe Noboru Kashiwagi |
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Affiliation: | (1) Department of Transplantation Immunology, Kitasato University School of Medicine, 1-15-1 Kitasato, 228 Sagamihara, Japan;(2) Present address: The Second Laboratory of Bacteriology, The Kitasato Institute, 5-9-1 Shirogane, Minato-ku, 108 Tokyo, Japan |
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Abstract: | Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401 (DRw14-Dw9) and DRB1*1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) was amplified enzymatically and sequenced after cloning intto a plasmid vector. The amino acid sequence of the first domain in the DR1 chain encoded in the DRB1-14c allele was more similar to that of the DRB1*1401 allele (three amino acid substitutions).than to that of the DRB1*1402 allele (six amino acid substitutions). No polymorphic amino acid residue that could explain the common serologic HLA-DRw14 specificity was identified among the sequences of the three DRw14-related alleles. Sequence-specific oligonucleotides (SSOs) were synthesized on the basis of the DRB1-14c nucleotide sequence and used for genotyping of the Japanese population. These SSOs served as useful probes for identifying the DRB1-14c allele in a wide range of donors.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M33693. |
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