|
|||||
|
|
Direct fractionation of genes by preparative electrophoresis of Bacillus subtilis DNA |
|
| Authors: | K F Bott C P Moran M H Edgell D Bentley L Charles |
| Affiliation: | Department of Bacteriology and Curriculum in Genetics, University of North Carolina, Medical School. Chapel Hill, NC 27514 U.S.A. |
| Abstract: | Discontinuous electrophoresis through agarose has been shown to be a satisfactory method for preparation of biologically active restriction fragments from milligram quantities of DNA. The DNA is obtained in sufficient quantity for: (1) direct use in genetic transformation, (2) the production of multiple-dimensional restriction analyses, or (3) use as a high-resolution hybridization probe. |
| Keywords: | Recombinant DNA gene cloning restriction endonucleases gene enrichment E buffer 40 mM tris-hydroxylaminomethane 20 mM Na acetate ethylenediamine tetraacetate adjusted to pH 7.2 with glacial acetic acid SDS sodium dodecyl sulfate SSC 0.15 M NaCl citrate pH 7.6 |
| 本文献已被 ScienceDirect 等数据库收录！ | |