Caffeine-induced uncoupling of mitosis from DNA replication in tobacco BY-2 cells |
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| Authors: | Amino Shin-ichi Nagata Toshiyuki |
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| Institution: | (1) Present address: Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Minamisawa, Minami-ku, 005 Sapporo, Japan;(2) Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | Caffeine induced a mitosis-like state in cultured tobacco (Nicotiana tabacum L.) BY-2 cells after DNA synthesis had been arrested by aphidicolin. Cells were synchronized upon removal of aphidicolin.
When aphidicolin was readded, the cell cycle was again interrupted and caffeine, when added with aphidicolin, induced the
mitosis-like state in 5–10% of cells. |
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| Keywords: | Caffeine Cell cycle Cultured tobacco cell BY-2 Mitosis Premature chromosome condensation (PCC) |
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