| 蜘蛛杀虫肽在大肠杆菌中的表达及其活性分析 |
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| 引用本文： | 蒋红,朱玉贤,陈章良. 蜘蛛杀虫肽在大肠杆菌中的表达及其活性分析[J]. 中国生物化学与分子生物学报, 1996, 12(3): 295-298 |
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| 作者姓名： | 蒋红 朱玉贤 陈章良 |
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| 作者单位： | 北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室 |
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| 基金项目： | 澳大利亚Deakin公司,北京大学蛋白质工程及植物基因工程国家重点实验室资助 |
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| 摘 要： | 以Ｔ７为启动子构建了蜘蛛杀虫肽基因的表达质粒ｐＴＨＩ９，转化大肠杆菌ＢＡＬ３１，在其对数生长期加入１ｍｍｏｌ／ＬＩＰＴＧ能诱导杀虫肽的产生，表达产物占菌体总蛋白的１３．７５％，并且表达的杀虫肽具有功能活性．
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| 关 键 词： | 蜘蛛杀虫肽 基因表达 昆虫毒性 |
| 收稿时间： | 1996-06-20 |
Expression of the Spider Insecticidal Peptide in Escherichia coli and Activity Assay |
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| Jiang Hong,Zhu Yu-Xian,Chen Zhang-Liang. Expression of the Spider Insecticidal Peptide in Escherichia coli and Activity Assay[J]. Chinese Journal of Biochemistry and Molecular Biology, 1996, 12(3): 295-298 |
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| Authors: | Jiang Hong Zhu Yu-Xian Chen Zhang-Liang |
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| Affiliation: | (The National Laboratory of Plant Genetic Engineering and Protein Engineering, College of Life Sciences, Peking University, Beijing 100871 |
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| Abstract: | The synthesized gene encoding insecticidal peptide was inserted into expression vector pET11c and controlled by T7 promoter. The recombinant plasmid pTHI9 was transformed into E.coli BAL 31 and induced by addition of 1 mmol/L IPTG into the culture at logarithmic phase. Analysis by SDS-PAGE and Western-blot revealed that the insecticidal peptide was produced by inducement. The product made up 13. 75% of the total bacteria proteins and proved to be active via assay of insect toxicity. |
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| Keywords: | Spider insecticidal peptide Gene expression Insect toxicity |
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