Essential role of an adenylate cyclase in regulating Vibrio vulnificus virulence |
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Authors: | Kim Young Ran Kim Soo Young Kim Choon Mee Lee Shee Eun Rhee Joon Haeng |
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Institution: | Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju 501-746, South Korea. |
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Abstract: | Vibrio vulnificus, a halophilic estuarine bacterium, causes a fatal septicemia and necrotizing wound infection. To investigate the role of cAMP in V. vulnificus virulence regulation, an in-frame deletion mutant of the cya gene encoding adenylate cyclase was constructed. The cya null mutation resulted in a pleiotropic change of virulence phenotypes. The production of hemolysin and protease, the motility, and the cytotoxicity were decreased by the cya mutation. The defects in the cya mutant were functionally complemented in trans by a plasmid carrying the wild type cya allele. The V. vulnificus cya mutant exhibited a 100-fold increase in LD50 to mice. The result indicates that cAMP plays an essential role in the global regulation of V. vulnificus virulence. |
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Keywords: | Vibrio vulnificus cya Cytotoxicity Motility Global virulence regulator |
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