Genetic diversity and bottleneck studies in the Marwari horse breed |
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Authors: | A K Gupta M Chauhan S N Tandon Sonia |
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Institution: | (1) National Research Centre on Equines, Sirsa Road, 125 001 Hisar, India;(2) Guru Jambeshwar University, 125 001 Hisar, India |
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Abstract: | Genetic diversity within the Marwari breed of horses was evaluated using 26 different microsatellite pairs with 48 DNA samples
from unrelated horses. This molecular characterisation was undertaken to evaluate the problem of genetic bottlenecks also,
if any, in this breed. The estimated mean (± s.e.) allelic diversity was 5.9 (± 2.24), with a total of 133 alleles. A high
level of genetic variability within this breed was observed in terms of high values of mean (±s.e.) effective number of alleles
(3.3 ± 1.27), observed heterozygosity (0.5306 ± 0.22), expected Levene’s heterozygosity (0.6612 ± 0.15), expected Nei’s heterozygosity
(0.6535 ± 0.14), and polymorphism information content (0.6120 ± 0.03). Low values of Wright’s fixation index, FIS (0.2433 ± 0.05) indicated low levels of inbreeding. This basic study indicated the existence of substantial genetic diversity
in the Marwari horse population. No significant genotypic linkage disequilibrium was detected across the population, suggesting
no evidence of linkage between loci. A normal ‘L’ shaped distribution of mode-shift test, non-significant heterozygote excess
on the basis of different models, as revealed from Sign, Standardized differences and Wilcoxon sign rank tests as well as
non-significantM ratio value suggested that there was no recent bottleneck in the existing Marwari breed population, which is important information
for equine breeders. This study also revealed that the Marwari breed can be differentiated from some other exotic breeds of
horses on the basis of three microsatellite primers. |
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Keywords: | microsatellite genetic diversity bottleneck Marwari horse |
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