The cytotoxic eosinophil cationic protein (ECP) has ribonuclease activity |
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| Authors: | U Gullberg B Widegren U Arnason A Egesten I Olsson |
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| Affiliation: | 1. Division of Hematology, Department of Medicine, University of Lund, S-221 85 Lund, Sweden;2. Department of Genetics, University of Lund, S-223 62 Lund, Sweden;1. Department of Molecular, Cellular, and Developmental Biology, Ann Arbor, Michigan, 48109, USA;2. Program in Neuroscience, University of Michigan, Ann Arbor, Michigan, 48109, USA;1. Department of Chemical and Pharmaceutical Sciences, University of Trieste, P.le Europa 1/via L. Giorgieri 1, 34127 Trieste, Italy;2. Department of Pharmacy and BioTechnology, University of Bologna, Via S. Donato 19/2, 40127 Bologna, Italy;3. Department of Chemistry and NIS Centre, University of Torino, V. Giuria 7, 10125 Torino, Italy;4. Leicester School of Pharmacy, De Montfort University, The Gateway, LE1 9BH Leicester, United Kingdom;5. Elettra – Sincrotrone Trieste, S.S. 14 Km 163.5 in Area Science Park, 34149 Basovizza, Trieste, Italy;6. Helminth Drug Development Unit, Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstr. 57, CH-4051 Basel, Switzerland;7. Aix-Marseille Université, CNRS, ICR (UMR 7273), 13397 Marseille cedex 20, France;1. Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada;2. Department of Pathology, Dalhousie University, Halifax, Canada;3. Immunology Program and Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA;4. Department of Medicine, Weill Cornell Medical College, New York, NY, USA;1. Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0202, USA;2. Faculty of Science and Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan;3. Wildlife Fish and Conservation Biology, University of California Davis, One Shields Avenue, Davis, CA 95616-8627, USA;4. Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan;1. Stowers Institute for Medical Research, Kansas City, MO 64110, United States;2. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, United States |
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| Abstract: | The eosinophil cationic protein (ECP) is a specific cytotoxic constituent of granules. In this work we demonstrated that ECP has a ribonuclease activity. Purified ECP was resolved by ion exchange chromatography into subfractions, which all showed ribonuclease activity. Another eosinophil granule protein, EPX, identical with eosinophil-derived neurotoxin (EDN) had a 125-fold higher RNase activity than ECP. ECP may exert its cytotoxic effects on parasites and cells because of its extreme basicity alone or it may be internalized and act by degrading mRNA. |
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