Intracellular free [Ca2+] in circulating lymphocytes of spontaneously hypertensive rats |
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Authors: | G Bruschi M E Bruschi M Caroppo G Orlandini C Pavarani A Cavatorta |
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Institution: | Istituto di Clinica Medica e Nefrologia, Università di Parma, Via Gramsci, 14 - 43100 PARMA-ITALY |
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Abstract: | In the light of previous reports suggesting a common abnormality of Ca handling in most tissues of hypertensive humans and rats, we applied a novel technique using the fluorescent probe Quin 2 for measurement of cytosolic free Ca2+ in lymphocytes of spontaneously hypertensive rats (SHR). (Ca2+)i is increased in SHR (122.1 +/- 7.4 nM) versus normotensive Wistar-Kyoto (WKY) control rats (81.1 +/- 6.3 nM) Membrane exchange, as challenged by varying the extracellular Ca concentration over a 10(5)-fold range proved to be relatively unimportant in regulating (Ca2+)i and did not significantly affect the difference between SHR and WKY. Catecholamines and ouabain had no appreciable effect on (Ca2+)i. The mechanisms of increased (Ca2+)i in SHR lymphocytes remain to be fully elucidated. |
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