The role of biotin in carbohydrate metabolism of Saccharomyces cerevisiae |
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| Authors: | MOAT A G LICHSTEIN H C |
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| Affiliation: | 1. Department of Biology, Boston College, Chestnut Hill, MA, USA;2. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA;3. Graduate Program in Immunology, Tufts Graduate School of Biomedical Sciences, Boston, MA, USA;4. Stuart B. Levy Center for Integrated Management of Antimicrobial Resistance, Tufts Medical Center, Boston, MA, USA;5. Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA;6. Harvard Medical School, Boston, MA, USA;1. Industrial & Financial Mathematics Research Group, Department of Mathematics, Institut Teknologi Bandung, Jl. Ganesa 10 Bandung 40132, Indonesia;2. Department of Mathematics, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10 Makassar 90245, Indonesia |
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| Abstract: | The investigations of the effect of biotin deficiency upon carbohydrate metabolism by washed cell suspensions of Saccharomyces cerevisiae 139 show that biotin stimulates the rate of fermentation and oxidation of carbohydrates by deficient cells. Aspartic acid partially replaces biotin in stimulating the rate of fermentation and oxidation of glucose by biotin-deficient yeast. Biotin or biotin plus aspartic acid elicits a greater response than aspartic alone in the stimulation of glucose metabolism by yeast, suggesting roles for biotin in both fermentation and oxidation other than its role in the metabolism of aspartic acid.The rate of adaptation of biotin-deficient cell suspensions of S. cerevisiae to sucrose fermentation is much more rapid in the presence of biotin, suggesting that this vitamin is concerned in the synthesis of the adaptive enzymes necessary for fermentation of this sugar.It has been found that cell-free extracts with high fermentative ability can be obtained if the synthetic medium is supplemented with 0.5% casein hydrolyzate. |
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