松材线虫对其携带的细菌繁殖的影响

1.引言松材线虫病（Bursaphelenchus xylophilus）是松树的一种毁灭性病害,在日本、中国、韩国和北美、尼日利亚和葡萄牙等国家蔓延,造成了巨大经济损失,其中以日本和中国受害最重.一直认为松材线虫是引起该病的唯一病原,但近十几年来的研究发现,细菌在致病过程中可能起着重要作用,相继从病木和松材线虫体上分离到能对黑松苗有致萎活性的细菌.赵博光等首次根据实验提出松材线虫病是线虫和细菌共同侵染引起的复合侵染病害的假说,并在以后的试验中得到了验证.关于松材线虫对其细菌繁殖的影响研究鲜有报道.本试验采用从感病松树上分离并鉴定了的细菌菌株中选取假单胞属7株、其它属的细菌菌株3株,

Effects of pine wood nematode on propagation of its carrying bacteria

In this paper,the aseptic eggs of Bursaphelenchus xylophilus were obtained after treated with 30% H_2O_2,and cultured with Pinus thunbergii callus.Ten B.xylophilus-carrying bacterial strains directly isolated from diseased P.thugbergii and P.massoniana in six epidemic provinces i.e.,GcM6-2A Pseudomonas putida,GcM6-1A P.putida,ZpB1-2A P.putida,HeM2A Pseudomonas sp.,HeM1A Pseudomonas sp.,HeM142B Pseudomonas sp.,GcM1-3A P.cepacia and HM3 Pantoeu sp.,ZpB4-2B Staphylococcus sciuri and ZpB2-3A Enterobacter amnigenus,were collected,and the effects of axenic B.xylophilus (ABx) on their propagation were studied.The results showed that pine wood nematode (PWN) promoted the propagation of 7 bacterial strains in Pseudomonas and 1 bacterial strain in Pantoeu sp.,including Pseudomonas putida,P.putida,P.putida,Pseudomonas sp.,Pseudomonas sp.,Pseudomonas sp.,P.cepacia and Pantoeu sp.,but inhibited Staphylococcus sciuri and Enterobacter amnigenus,which could explain the phenomenon that Pseudomonas was the prevailing genus of the bacteria carried by PWN,and might provide essential nutrition to the bacteria.The close relationship between PWN and bacterial strains in Pseudomonas might account for the pine wood nematode disease.