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Modelling real-time simultaneous saccharification and fermentation of lignocellulosic biomass and organic acid accumulation using dielectric spectroscopy
Authors:Bryant David N  Morris Stephen M  Leemans David  Fish Steven A  Taylor Stephen  Carvell John  Todd Robert W  Logan Daniel  Lee Matthew  Garcia Naroa  Ellis Andrew  Gallagher Joe A
Institution:Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK.
Abstract:Dielectric spectroscopy (DS) is routinely used in yeast and mammalian fermentations to quantitatively monitor viable biomass through the inherent capacitance of live cells; however, the use of DS to monitor the enzymatic break down of lignocellulosic biomass has not been reported. The aim of the current study was to examine the application of DS in monitoring the enzymatic saccharification of high sugar perennial ryegrass (HS-PRG) fibre and to relate the data to changes in chemical composition. DS was capable of both monitoring the on-line decrease in PRG fibre capacitance (C=580 kHz) during enzymatic hydrolysis, together with the subsequent increase in conductivity (G=580 kHz) resulting from the production of organic acids during microbial growth. Analysis of the fibre fractions revealed >50% of HS-PRG lignocellulose had undergone enzymatic hydrolysis. These data demonstrated the utility of DS biomass probes for on-line monitoring of simultaneous saccharification and fermentation (SSF).
Keywords:DS  dielectric spectroscopy  HS-PRG  high sugar perennial rye grass  SHF  separate hydrolysis and fermentation  SSF  simultaneous saccharification and fermentation  HPLC  high performance liquid chromatography
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