Genetic basis of sodium exclusion and sodium tolerance in yeast. A model for plants |
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Authors: | Rosario Haro,Marí a A. Bañ uelos,Francisco J. Quintero,Francisco Rubio,Alonso Rodrí guez-Navarro |
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Affiliation: | Laboratorio de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, E-28040 Madrid, spain. |
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Abstract: | A yeast strain carrying disruptions in TRK1 and ENA genes was very sensitive to Na+ because uptake discriminated poorly between K+ and Na+, and Na+ efflux was insignificant. Transformation with TRK1 and ENA1 restored discrimination, Na+ efflux and Na+ tolerance. Increasing external Ca2+ increased Na+ tolerance almost in the same proportion in TRK1 enal cells and in trkl ENAI cells, suggesting an unspecific effect of this cation. By using a vacuolar ATPase mutant, the role of the vacuole in Na+ tolerance was also demonstrated. The yeast model of Na+ exclusion and Na+ tolerance may be extended to plants. |
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Keywords: | Ion transport Saccharomyces cerevisiae sodium exclusion sodium tolerance yeast |
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