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Cloning of a Resistance Gene Analog from Wheat and Development of a Codominant PCR Marker for Pm21
Authors:Ya-Ping Chen  Hua-Zhong Wang  Ai-Zhong Cao  Chun-Mei Wang  Pei-Du Chen
Institution:(State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University;, Nanjing 210095, China)
Abstract:To investigate the mechanism of resistance to wheat (Triticum aestivum L.) powdery mildew, suppression subtractive hybridization was conducted between an isogenic resistant line carrying Pm21 and its recurrent parent Yangmai 5 to isolate the resistance relative genes. A cDNA fragment specifically expressed in the resistant line was obtained and its full length was cloned by in silico cloning and RT-PCR. This gene encoded a deduced protein of 219 amino acids with a leucine-rich repeat (LRR) motif, often found in plant resistance genes, and was designated as Ta-LRR2. Ta-LRR2 had an increased expression level in the resistant line after inoculation with Erysiphe graminis DC. f. sp. tritici Marchal. PCR analysis with different cytogenetic stocks suggested that Ta-LRR2 was specifically associated with chromosome arms 6VS and 6AS. Linkage analysis further showed that Ta-LRR2 could be used as a resistance gene analog polymorphism marker of Pm21 for marker-assisted selection in germplasm enhancement and breeding practice.Moreover, how to isolate Pm21 based on the information obtained for Ta-LRR2 is discussed.
Keywords:chromosome assignment  molecular marker  suppression subtractive hybridization (SSH)  Ta-LRR2  Triticum aestivum-Haynaldia villosa 6VS/6AL translocation line
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