Cloning of a Resistance Gene Analog from Wheat and Development of a Codominant PCR Marker for Pm21 |
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Authors: | Ya-Ping Chen Hua-Zhong Wang Ai-Zhong Cao Chun-Mei Wang Pei-Du Chen |
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Institution: | (State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University;, Nanjing 210095, China) |
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Abstract: | To investigate the mechanism of resistance to wheat (Triticum aestivum L.) powdery mildew, suppression subtractive hybridization was conducted between an isogenic resistant line carrying Pm21 and its recurrent parent Yangmai 5 to isolate the resistance relative genes. A cDNA fragment specifically expressed in the resistant line was obtained and its full length was cloned by in silico cloning and RT-PCR. This gene encoded a deduced protein of 219 amino acids with a leucine-rich repeat (LRR) motif, often found in plant resistance genes, and was designated as Ta-LRR2. Ta-LRR2 had an increased expression level in the resistant line after inoculation with Erysiphe graminis DC. f. sp. tritici Marchal. PCR analysis with different cytogenetic stocks suggested that Ta-LRR2 was specifically associated with chromosome arms 6VS and 6AS. Linkage analysis further showed that Ta-LRR2 could be used as a resistance gene analog polymorphism marker of Pm21 for marker-assisted selection in germplasm enhancement and breeding practice.Moreover, how to isolate Pm21 based on the information obtained for Ta-LRR2 is discussed. |
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Keywords: | chromosome assignment molecular marker suppression subtractive hybridization (SSH) Ta-LRR2 Triticum aestivum-Haynaldia villosa 6VS/6AL translocation line |
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