Influence of strain and cultivation procedure on the performance of simultaneous saccharification and fermentation of steam pretreated spruce |
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| Institution: | 1. Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, Thailand;2. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;3. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand;4. Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand;1. Department of Chemistry, West Bengal State University, Barasat, Kolkata 700126, India;2. Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Física, Facultade de Química, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain, Spain;3. Department of Chemistry, University of Pune, India;1. Clinical Trials Center, Cardiovascular Research Foundation, New York, New York;2. Department of Cardiology, Asan Medical Center, Seoul, South Korea;3. Division of Cardiology, Columbia University Medical Center, New York, New York;4. Department of Cardiology, Shaare Zedek Medical Center, Jerusalem, Israel;5. Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, New York;6. Antwerp Cardiovascular Center, ZNA Middelheim, Antwerp, Belgium;7. Thoraxcenter, Rotterdam, The Netherlands;1. Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9L 0G2, Canada;2. Trent School of The Environment, Trent University, Peterborough, ON K9L 0G2, Canada;1. Department of Chemistry, R.A. Arts, Shri, M. K. Commerce and Shri, S. R. Rathi Science College, Washim 444505, India;2. Department of Chemistry, Savitribai Phule Pune University, Pune 411007, India;3. Nano and Computational Materials Lab, Catalysis Division, CSIR-National Chemical Laboratory, Pune 411008, India;4. Present Address: Functional Materials Division, CSIR-Central Electrochemical Research Institute, Karaikudi 630006, India |
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| Abstract: | Yeast to be used in simultaneous saccharification and fermentation (SSF) of lignocelluloses materials has to be prepared in a separate cultivation step. The effects of the cultivation procedure on the performance of SSF of steam pretreated softwood were studied in the current work. The yeast used in the SSF was either directly commercially available Baker's yeast (as packaged yeast) or the same strain of yeast produced from the hydrolysate obtained in the pretreatment of the softwood material. A second strain of Saccharomyces cerevisiae TMB3000, isolated from spent sulphite liquor, was also compared with the commercial Baker's yeast. The strains were tested in SSF at substrate loads of 3, 5 and 8% dry weight of water insoluble material. Final ethanol yields were above 85% of the theoretical (based on the available hexoses) in all cases, except for the package yeast for the 8% substrate load, in which case the final yield was less than 65%. The cultivation procedure was found to have a significant impact on the performance during SSF, as well as in small-scale fermentations of hydrolysate liquor without solid material. The Baker's yeast cultivated on the hydrolysate from the steam pretreatment had in all cases a higher productivity, in particular at the highest substrate load. Cultivated Baker's yeast had a slightly higher productivity than TMB3000. The results suggest that the adaptation of the yeast to the inhibitors present in the medium is an important factor that must be considered in the design of SSF processes. |
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