Effect of different carbon and nitrogen sources on laccase and peroxidases production by selected Pleurotus species |
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| Affiliation: | 1. Institute of Botany, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia and Montenegro;2. Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, P. O. Box 12, Rehovot 76100, Israel;3. Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel;1. Department of Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;2. Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;1. College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian 350116, China;2. Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou, Fujian 350116, China;3. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China;1. Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca, Morelos CP 62209, Mexico;2. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Chamilpa, Cuernavaca, Morelos CP 62210, Mexico;3. Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca, Morelos CP 62209, Mexico;1. Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111 Algiers, Algeria;2. Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia;3. National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA) 11, Bd Amirouche PO Box 67, Bou Ismaïl, 42415, Tipaza, Algeria;4. Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria;1. Microorganism Fermentation Engineering and Technology Research Center of Anhui Province, Anhui Polytechnic University, Central Beijing Road, Wuhu 241000, China;2. School of Biological and Chemical Engineering, Anhui Polytechnic University, Central Beijing Road, Wuhu 241000, China;1. Biotechnology Department, Graduate School of Natural and Applied Sciences, Dokuz Eylül University, 35160 Buca, Izmir, Turkey;2. Chemistry Department, Biochemistry Division, Faculty of Science, Dokuz Eylül University, 35160 Buca, Izmir, Turkey |
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| Abstract: | Pleurotus eryngii, P. ostreatus and P. pulmonarius produced laccase (Lac) both under conditions of submerged fermentation (SF) and solid-state fermentation (SSF) with all of the investigated carbon and nitrogen sources. The highest levels of Lac activity were found in P. eryngii, under SF conditions of dry ground mandarine peels and in P. ostreatus, strain No. 493, under SSF conditions of grapevine sawdust.High levels of peroxidases activities were occurred in P. ostreatus, strain No. 494, and P. pulmonarius, under SSF conditions of grapevine sawdust, whereas in SF, these activities were either very low or absent.After purification of extracellular crude enzyme mixture of investigated species and strain which were grown in the medium with the best carbon sources, the Lac activity measurements revealed two peaks in P. eryngii, three peaks in both P. ostreatus strains and three in P. pulmonarius. Results obtained after purification also showed that the levels of phenol red oxidation in absence of external Mn2+ were higher than phenol red oxidation levels in presence of external Mn2+.In the medium with the best carbon sources (mandarine peels and grapevine sawdust, respectively), both P. eryngii and P. ostreatus, strain No. 493, showed the highest Lac activity with (NH4)2SO4, as a nitrogen source, with a nitrogen concentration of 20 and 30 mM, respectively.In P. ostreatus, strain No. 494, and P. pulmonarius, the best nitrogen sources for peroxidases production were peptone in a concentration of 0.5% and NH4NO3 with a nitrogen concentration of 30 mM, respectively. |
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