Immunolocalization of a defense-related 87 kDa cystatin in leaf blade of tomato plants |
| |
| Affiliation: | 1. Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-600 Campos dos Goytacazes, Rio de Janeiro, Brazil;2. Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-600 Campos dos Goytacazes, Rio de Janeiro, Brazil;1. Centro de Biotecnologia & Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;2. John Innes Centre, Norwich, United Kingdom;3. Centro de Ciências Biológicas e da Saúde & Programa de Pós-Graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brazil;1. Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, 550025, China;2. Guizhou Minzu University, Guiyang, 550025, China;3. College of Forestry, Guizhou University, Guiyang, 550025, China;1. Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huai’an 223300, Jiangsu, China;2. Huaian Key Laboratory of Food Components and Functional Foods, Huaiyin Normal University, Huai’an 223300, Jiangsu, China;3. College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, China;1. Department of Horticultural Science, Imam Khomeini International University, Qazvin, 34148-96818, Iran;2. Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, 51664, Iran;3. Zhejiang University, College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agri-Food Processing, Hangzhou, 310058, People’s Republic of China;4. Ningbo Research Institute, Zhejiang University, Ningbo, 315100, People’s Republic of China;5. Fuli Institute of Food Science, Hangzhou, 310058, People’s Republic of China;1. Department of Horticultural Science, Imam Khomeini International University, Qazvin, 34148-96818, Iran;2. Zhejiang University, College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agri-Food Processing, Hangzhou, 310058, People’s Republic of China;3. Ningbo Research Institute, Zhejiang University, Ningbo, 315100, People’s Republic of China;4. Fuli Institute of Food Science, Hangzhou, 310058, People’s Republic of China;1. Programa de Investigación de Producción de Biomoléculas, Unidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México. Ciudad de México, México;2. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, México;3. Unidad de Microscopía Electrónica, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor., México |
| |
| Abstract: | In our previous work, we described the defensive potential of a wound- and methyl jasmonate-inducible 87 kDa tomato cystatin and its accumulation in a crystalline form. Here, we report the immunolocalization of this cysteine proteinase inhibitor in tomato leaf blade. Methyl jasmonate treated wild type plants showed accumulation of crystalline structures that were specifically and strongly stained with polyclonal antibodies against tomato cystatin. Crystalline cystatin was found in palisade and spongy parenchyma cells and immuno-gold electron microscopy analysis revealed that the crystals were compartmentalized in the cytosol. The same pattern in localization of cystatin was observed in transgenic tomato plants superexpressing prosystemin transgene. Our data showing the accumulation of cystatin in response to methyl jasmonate and in response to a overproduction of a wound signal corroborate the protective role of this inhibitor. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
