Organic or mineral nitrogen source during Penicillium camembertii growth on a glucose limited medium |
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| Affiliation: | 1. Department of Biochemistry, Universidade Estadual de Maringa, Maringa, PR 87020-900, Brazil;2. Department of Biochemistry and Molecular Biology, Universidade Federal do Parana, P.O. Box 19046, Curitiba, PR 81531-990, Brazil;1. Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;2. Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark;3. Ningbo Academy of Inspection and Quarantine of the P.R. China, Ningbo 315012, China;4. Departement of Food Science, University of Liège – FARAH-Veterinary Public Health, Quartier Vallée 2, Avenue de Cureghem 10, Sart Tilman B43bis, 4000 Liège, Belgium;5. College of Food Science and Technology, Shanghai Engineering Research Centre of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | Penicillium camembertii was cultivated on a carbon-limited medium (glucose). Two nitrogen sources were compared, a mineral, ammonium, and an organic nitrogen source, lysine. Among the amino acids convenient nitrogen sources for P. camembertii, lysine was chosen since it cannot be assimilated as a carbon source for cell biosynthesis. During culture on glucose and ammonium, a decline phase immediately followed growth after glucose depletion, since no energy source remained in the medium. On the contrary, on glucose and lysine, a stationary state was recorded after glucose depletion, since lysine was used as the energy supply for cell maintenance, leading to the release of the corresponding carbon as CO2, while nitrogen from lysine was released as ammonium. |
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