Ribosomal DNA (rDNA) spacer polymorphism in mole rats |
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Authors: | Suzuki H; Moriwaki K; Nevo E |
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Institution: | National Institute of Genetics, Japan. |
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Abstract: | Genetic differentiation of ribosomal DNA (rDNA) nontranscribed-spacer (NTS)
polymorphism was analyzed in 50 individuals from 13 populations among the
four chromosomal species (2n = 52, 2n = 54, 2n = 58, and 2n = 60) of
subterranean mole rats of the Spalax ehrenbergi complex in Israel. Southern
blot analysis with a mouse rDNA probe and two restriction enzymes, EcoRI
and BamHI, revealed various sizes of major restriction fragments. The
assumption that this variation is due to length polymorphism of NTS DNA was
supported by the construction of restriction-site maps. On the basis of the
EcoRI and BamHi fragment lengths, we could characterize the major types of
NTS rDNA repeating units in each individual. Each member of the central
population in the four chromosomal groups of mole rats has a characteristic
combination of the NTS types, suggesting that the karyotype groups were
genetically diverged. Some near-hybrid-zone populations reflect
similarities with the rDNA spectra of a neighbor chromosomal group. This
might have resulted from gene flow across the hybrid zones.
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