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Large-Scale isolation of acid phosphatase from human erythrocytes |
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| Authors: | R. A. Fisher H. Harris M. Houldsworth M. D. Lilly P. Dunnill |
| Abstract: | A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 27%. Results for the type A enzyme are also presented. |
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