Cloning and expression in Escherichia coli of a species-specific Chlamydia trachomatis outer membrane antigen |
| |
| Authors: | Ravi Kaul Wanda M Wenman |
| |
| Institution: | Division of Infectious Diseases, Department of Pediatrics, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| |
| Abstract: | Abstract A gene library of Chlamydia trachomatis serovar L2 (strain 434) was constructed in Escherichia coli using plasmid pBR322. Amongst 200 recombinants we have identified and characterized a recombinant E. coli that expresses a protein antigen of M r 74 000 similar in size to an outer membrane antigen produced by elementary bodies of C. trachomatis . Immunologically, the molecule synthesised by E. coli has the same specificity as the protein encoded by serovar L2. A 1.8 kb DNA fragment from the recombinant insert, used as a hybridization probe, confirmed the species specificity of this clone at the gene level. |
| |
| Keywords: | Gene library chlamydiae E coli RR1 plasmid pBR322 recombination |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
