Distribution and characterization of neuropeptide Y-like immunoreactivity in the brain and pituitary of the goldfish |
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Authors: | A Pontet J M Danger P Dubourg G Pelletier H Vaudry A Calas Dr O Kah |
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Institution: | (1) Laboratoire de Physiologie des Interactions Cellulaires, UA CNRS 339, Université de Bordeaux I, Talence, France;(2) Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Université de Rouen, Mont-Saint-Aignan, France;(3) MRC Group in Molecular Endocrinology, CHU Laval, Québec, Canada;(4) Laboratoire de Physiologie Cellulaire et Comparée, Parc Valrose, F-06034 Nice Cedex, France |
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Abstract: | Summary The distribution of neuropeptide Y (NPY) immunoreactivity has been studied by means of immunocytochemistry and radioimmunoassay in the brain of the goldfish. It was found that NPY had a widespread distribution in the entire brain in particular in the telencephalon, diencephalon, optic tectum and rhombencephalon. In the pituitary gland, positive type-B fibers were observed in the various lobes frequently in direct contact with secretory cells, in particular the gonadotrophs, somatotrophs and MSH (melanocyte-stimulating hormone) secreting cells. When measured by radioimmunoassay, the highest NPY concentrations were found in the pituitary and telencephalon, confirming the results of immunocytochemistry. The displacement curves obtained with serial dilutions of brain extracts were parallel to that of synthetic porcine NPY. Following high performance liquid chromatography, the NPY-like material extracted from goldfish brain co-eluted as a single peak with synthetic porcine NPY. These data demonstrate the presence of an NPY-like substance widely distributed in the goldfish brain. The observation of NPY-immunoreactive fibers in the pituitary gland suggests that, among its other functions, NPY may play a role in the neuroendocrine regulation of pituitary function. |
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Keywords: | Neuropeptide Pituitary Brain Immunocy tochemistry HPLC Carassius auratus (Teleostei) |
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