Cloning of bacteriophage PM2 DNA in Escherichia coli K12. |
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Authors: | B Rempola M Fikus |
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Affiliation: | (1) Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36, 02-532 Warszawa, Poland |
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Abstract: | Summary DNA fragments of phage PM2 restricted with HindIII endonuclease was cloned in the vector pBR 322 in an Escherichia coli K 12 host. The attempt to clone full length PM2 DNA restricted with PstI endonuclease has been unsuccesful. From six randomly chosen recombinant clones DNA was purified and analysed with EcoRI, PstI and HindIII endonucleases. The physical map of three chimeric plasmids was unequivocally established. It was shown, that the whole PM2 genome was cloned, although in separate fragments. However, most of the recombinant clones were instable in the absence of selective pressure. |
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