Regulation of L-arabinose transport in Salmonella typhimurium LT2 |
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Authors: | Jar-How Lee Robert J. Russo Laurel Heffernan Gary Wilcox |
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Affiliation: | (1) Department of Microbiology, College of Letters and Science, University of California, 90024 Los Angeles, California, USA;(2) Molecular Biology Institute, University of California, 90024 Los Angeles, California, USA |
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Abstract: | Summary The inducible L-arabinose transport system was characterized in Salmonella typhimurium LT2. Only one L-arabinose transport system with a Km of 2x10-4 M was identified. The results suggested that araE may be the only gene which codes for L-arabinose transport activity under the conditions tested. An araE-lac fusion strain was used to study the induction of the araE gene. No araE expression was detected when the L-arabinose concentration was lower than 1 mM. The expression of araE reached a maximum in the presence of 50 mM L-arabinose, and was significantly reduced in the presence of D-glucose. Expression of the araBAD and araE genes was coordinately regulated. The concentration of L-arabinose that allowed maximum araBAD gene expression was 50-fold lower in an araE+ strain compared to an araE strain. |
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