A developmental study of Glycine max cell and protoplast isolation in relation to leaf age and photosynthetic competence |
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Authors: | Richard T. Rees rew H. Cobb Kenneth E. Pallett |
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Affiliation: | Dept of Life Sciences, Trent Polytechnic, Burton Street, Nottingham NG1 4BU, U.K. |
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Abstract: | Leaf mesophyll cells were isolated from developing first trifoliate leaves of Glycine max (L.) Merr cv. Fiskeby V using a mechanical isolation procedure combined with low speed centrifugation. Cell yields of 17 ± 1.7% were routinely obtained with 55–75% intactness, as assessed by staining techniques, fluorescence transients and the ability of cells to convert to protoplasts after enzyme treatment. Rates of leaf photosynthesis were maximal in 27-day-old plants [280 μmol O2 evolved (mg chlorophyll)-1h-1], from which isolated cells and protoplasts gave rates of up to 140 μmol O2 evolved (mg chlorophyll)-1 h-1. Results are discussed in relation to leaf development and cell status during the attainment of photosynthetic competence. |
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Keywords: | Isolated cells photosynthesis soybean |
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