Production of human epidermal growth factor by an ampicillin resistant recombinant Escherichia coli strain |
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| Authors: | V B Yadwad S Wilson O P Ward |
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| Institution: | (1) Microbial Biotechnology Laboratory, Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
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| Abstract: | Summary The effect of medium composition and initial glucose concentration on production of hEGF by recombinant E. coli cells was investigated. Optimum hEGF production was observed in a yeast extract/acid hydrolysed casein/salts media containing an initial glucose concentration of 10 g.l-1. A maximum hEGF titer of 250 mg.l-1 was obtained in this medium after 32 h in laboratory fermenters with pH, temperature, agitation and aeration set at 6.8, 30°C, 500 rpm and 2 vvm, respectively. |
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