The inhibitory effect of [D-Arg1,D-Phe,D-Try7,9,Leu11] substance P on endothelin-1 binding sites in rat cardiac membranes. |
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| Authors: | X H Gu D J Casley W G Nayler |
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| Institution: | Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg VIC Australia. |
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| Abstract: | The specific binding of 125I]ET-1 to rat cardiac membrane fragments was inhibited by D-Arg1,D-Phe, D-Try7,9,Leu11] substance P substance P(D)], a potent bombesin antagonist. This inhibitory effect required high concentrations (greater than 3X10(-6)M) of substance P(D) and was accompanied by a steep increase in non-specific binding, and not a decrease in total binding. Such results indicate that substance P(D) does not competitively inhibit the specific binding of 125I]ET-1 to rat cardiac membrane fragments. |
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