A study of cryogenic tissue-engineered liver slices in calcium alginate gel for drug testing |
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Affiliation: | 1. General, Visceral, and Transplantation Surgery, Charité – Universitätsmedizin Berlin, Germany;2. Neonatology, Charité – Universitätsmedizin Berlin, Germany |
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Abstract: | To address issues such as transportation and the time-consuming nature of tissue-engineered liver for use as an effective drug metabolism and toxicity testing model, “ready-to-use” cryogenic tissue-engineered liver needs to be studied. The research developed a cryogenic tissue-engineered liver slice (TELS), which comprised of HepG2 cells and calcium alginate gel. Cell viability and liver-specific functions were examined after different cryopreservation and recovery culture times. Then, cryogenic TELSs were used as a drug-testing model and treated with Gefitinib. Cryogenic TELSs were stored at −80 °C to ensure high cell viability. During recovery in culture, the cells in the cryogenic TELS were evenly distributed, massively proliferated, and then formed spheroid-like aggregates from day 1 to day 13. The liver-specific functions in the cryogenic TELS were closely related to cryopreservation time and cell proliferation. As a reproducible drug-testing model, the cryogenic TELS showed an obvious drug reaction after treatment with the Gefitinib. The present study shows that the cryopreservation techniques can be used in drug-testing models. |
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Keywords: | Tissue-engineered liver slice Cryopreservation Calcium alginate gel HepG2 cell Drug-testing model |
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