Characterizing viscoelastic properties of breast cancer tissue in a mouse model using indentation |
| |
| Institution: | 1. State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China;2. Center for Molecular Imaging and Nuclear Medicine, School of Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, Jiangsu 215123, China;3. Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong 519000, China;4. Department of Radiology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215001, China;5. Department of Interventional Radiology & Vascular Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215001, China;6. Department of Surgical Oncology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215001, China;7. Department of Pathology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215001, China;1. Tissue Engineering and Microfluidics Laboratory, The Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, QLD, Australia;2. Division of Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, St Lucia, QLD, Australia;3. School of Chemical Engineering, University of Queensland, St Lucia, QLD, Australia;4. CSIRO, Division of Materials Science and Engineering, Clayton, Victoria, Australia;1. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China;2. Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;3. Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;4. Human Phenome Institute, Fudan University, Shanghai 201203, China;1. Institut Langevin, ESPCI ParisTech, PSL University, CNRS UMR7587, INSERM U979, Paris, France;2. Liver Unit, Hôpital Cochin, APHP, INSERM U1016, Université Paris-Descartes, Paris, France;3. Service d’anatomo-pathologie, Hôpital Cochin, APHP, INSERM U1016, Université Paris-Descartes, Paris, France;1. Shanghai Key Laboratory of Mechanics in Energy Engineering, Shanghai Institute of Applied Mathematics and Mechanics, School of Mechanics and Engineering Science Shanghai University, Shanghai 200444, China;2. Department of Mechanical Engineering, Michigan State University, East Lansing, MI 48824, USA |
| |
| Abstract: | Breast cancer is one of the leading cancer forms affecting females worldwide. Characterizing the mechanical properties of breast cancer tissue is important for diagnosis and uncovering the mechanobiology mechanism. Although most of the studies were based on human cancer tissue, an animal model is still describable for preclinical analysis. Using a custom-build indentation device, we measured the viscoelastic properties of breast cancer tissue from 4T1 and SKBR3 cell lines. A total of 7 samples were tested for each cancer tissue using a mouse model. We observed that a viscoelastic model with 2-term Prony series could best describe the ramp and stress relaxation of the tissue. For long-term responses, the SKBR3 tissues were stiffer in the strain levels of 4–10%, while no significant differences were found for the instantaneous elastic modulus. We also found tissues from both cell lines appeared to be strain-independent for the instantaneous elastic modulus and for the long-term elastic modulus in the strain level of 4–10%. In addition, by inspecting the cellular morphological structure of the two tissues, we found that SKBR3 tissues had a larger volume ratio of nuclei and a smaller volume ratio of extracellular matrix (ECM). Compared with prior cellular mechanics studies, our results indicated that ECM could contribute to the stiffening the tissue-level behavior. The viscoelastic characterization of the breast cancer tissue contributed to the scarce animal model data and provided support for the linear viscoelastic model used for in vivo elastography studies. Results also supplied helpful information for modeling of the breast cancer tissue in the tissue and cellular levels. |
| |
| Keywords: | Breast cancer Biomechanics Viscoelastic properties Indentation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
