Optimization of a protective medium for enhancing the viability of freeze-dried Bacillus amyloliquefaciens B1408 based on response surface methodology期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Optimization of a protective medium for enhancing the viability of freeze-dried Bacillus amyloliquefaciens B1408 based on response surface methodology

Institution:	1. Research and Development Center of Biorational Pesticides, Northwest A&F University, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, 22 Xinong Road, Yangling, Shaanxi 712100, China;2. Shaanxi Research Center of Biopesticide Engineering and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China;1. Shaanxi Research Center of Biopesticide Engineering and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China;2. Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China;3. School of Food and Biological Engineering, Shaanxi University of Science & Technology, Weiyang University Campus, Xi''an, Shaanxi 710021, China;1. Centre for Research & Evaluation, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India;2. PG Department of Biotechnology, Kongunadu Arts and Science College (Autonomous), Coimbatore, 641 029, India;3. Centre for Advanced Studies in Botany, University of Madras, Chennai, 600025, Tamil Nadu, India;4. School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, 613401, Tamil Nadu, India;1. Federal University of Santa Maria, Rural Sciences Center, Department of Science and Food Technology, 1000 Roraima Avenue, 97105-900, Santa Maria, RS, Brazil;2. Federal University of Santa Maria, Department of Food and Nutrition, 3751 Independence Avenue, 98300-000, Palmeira das Missões, RS, Brazil;3. Federal University of Bahia, Department of Preventive Veterinary Medicine and Animal Production, 500 Adhemar de Barros Avenue, 40170-115, Salvador, BA, Brazil;4. Federal University of Santa Maria, Natural and Exact Sciences Center, Department of Ecology and Evolution, 1000 Roraima Avenue, 97105-900, Santa Maria, RS, Brazil;5. Institute of Food Technology, Cereals and Chocolate Technology Center, 2880 Avenue Brazil, 13070-178, Campinas, SP, Brazil

Abstract:	Response surface methodology (RSM) is a commonly used system to optimize cryoprotectants of biocontrol strains when they are subjected to preparations. Various kinds of cryoprotectants and centrifugal conditions were tested to improve the survival of biocontrol agents after freeze-drying. To determine the optimum levels of incorporation of three cryoprotectants (glucose, trehalose and xylitol) in the freeze-drying process of strain Bacillus amyloliquefaciens B1408, a range of experiments based on Box-Behnken Design (BBD) were conducted. The results indicated that the suitable centrifugation conditions were 5000 r/min,10 min and the optimum concentrations of cryoprotectants were glucose 1.00%, trehalose 4.74% and xylitol 1.45%. The proven survival rate of cells after freeze-drying was 91.24%. These results convincingly demonstrated that freeze-drying could be used to preparation of biocontrol strain B1408. This study provides a theoretical basis for commercial possibilities and formulation development.

Keywords:	Biocontrol Freeze-drying Cryoprotectant Response surface methodology RSM"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "_":"Response surface methodology BBD"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"Box-Behnken Design B1408"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "$$":[{"#name":"italic" "_":"Bacillus amyloliquefaciens"} {"#name":"__text__" "_":" B1408 LB"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"Luria-Bertani medium CFUs"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"Colony-forming units
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