Comparison of knee injury threshold during tibial compression based on limb orientation in mice |
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| Institution: | 1. Biomedical Engineering Graduate Group, University of California, Davis, Davis, CA, United States;2. Department of Orthopaedic Surgery, University of California Davis Medical Center, Sacramento, CA, United States;1. School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA;2. Feinberg School of Medicine, Northwestern University, Chicago, IL, USA;3. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA;1. Department of Orthopaedics, Rutgers New Jersey Medical School, Newark, NJ, USA;2. Division of Foot and Ankle Surgery, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA;1. Institute of Biomedical Engineering, University of Oxford, United Kingdom;2. Department of Computer Science & INSIGNEO Institute for In Silico Medicine, University of Sheffield, United Kingdom;3. Mechanical Engineering and Materials Science, University of Pittsburgh, USA;1. Department of Orthopaedics, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada;2. Department of Orthopaedics, Kantonsspital Baselland, Rheinstrasse 26, 4410 Liestal, Switzerland;3. Department of Clinical Research, University Hospital Basel, Spitalstrasse 12, 4031 Basel, Switzerland;4. Department of Orthopaedics, University of Utah, 590 Wakara Way, Salt Lake City, UT 84108, USA |
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| Abstract: | Our previous studies used tibial compression overload to induce anterior cruciate ligament (ACL) rupture in mice, while others have applied similar or greater compressive magnitudes without injury. The causes of these differences in injury threshold are not known. In this study, we compared knee injury thresholds using a “prone configuration” and a “supine configuration” that differed with respect to hip, knee, and ankle flexion, and utilized different fixtures to stabilize the knee. Right limbs of female and male C57BL/6 mice were loaded using the prone configuration, while left limbs were loaded using the supine configuration. Mice underwent progressive loading from 2 to 20 N, or cyclic loading at 9 N or 14 N (n = 9–11/sex/loading method). Progressive loading with the prone configuration resulted in ACL rupture at an average of 10.2 ± 0.9 N for females and 11.4 ± 0.7 N for males. In contrast, progressive loading with the supine configuration resulted in ACL rupture in only 36% of female mice and 50% of male mice. Cyclic loading with the prone configuration resulted in ACL rupture after 15 ± 8 cycles for females and 24 ± 27 cycles for males at 9 N, and always during the first cycle for both sexes at 14 N. In contrast, cyclic loading with the supine configuration was able to complete 1,200 cycles at 9 N without injury for both sexes, and an average of 45 ± 41 cycles for females and 49 ± 25 cycles for males at 14 N before ACL rupture. These results show that tibial compression configurations can strongly affect knee injury thresholds during loading. |
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| Keywords: | Knee injury Tibial compression Osteoarthritis Bone adaptation Mechanical loading |
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